How to Remove Urine Smell From Concrete Subfloor
Remove any carpeting or non-adhesive flooring to expose the concrete subfloor.
Sweep the floor with a heavy-duty broom to remove loose dirt. Then vacuum the entire concrete subfloor using a shop vac.
Use a solution of a heavy-duty floor cleaner, bleach and warm water. Add ½ cup of bleach for each gallon of water.
Mop the floor in sections, changing the water when it is dirty. Continue mopping until the entire floor is cleaned.
Use the shop vac to pick up excess water. Let the floor dry overnight.
Mix 1 cup of white vinegar for each gallon of water. Apply this solution to the floor and let the floor dry.
Wait until the smell of the vinegar is gone. Check the area again for odors. Walk around the concreted area and mark the areas of odor with chalk.
Apply full strength vinegar to any area of the concrete floor that is still holding the odor. Let the vinegar dry. Repeat this step until all urine odor has dissipated.