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Summary: Starting with the first banjo string, tune it do a D. Learn to tune a banjo in standard G tuning with this free banjo video lesson from a professional banjo player.
"Tuning your banjo is very important. If you're not in tune you won't be able to sing along to your songs. Nothing will sound good and you can't play with other people. You need to know that this string which is your first string is a d. You can go over to a piano and find a d, hit the note and tune it correspondingly. This next one is a b, here is g, and a d. Your fifth string the one that is shorter than all the rest is another g. Now to tune your banjo you really only need to tune one string. If you can find, if you can tune this to d, the fifth fret on that string is the same as g of the next string. In a like manner, fourth fret on your g string is the same as the open string of the b. Third fret on the b string is the same as open on your first d string. If you go all the way to the twelfth fret on your g string it's the same as the fifth string g. Knowing this you only need to tune one string to get your whole banjo in tune."
eHow Article: Standard G Banjo Tuning