How to Inhibit Retinoic Acid
Retinoic acid, a metabolite of Vitamin A, is known for its ability to make cells grow and proliferate, stimulate development, and regulate embryonic development. Unfortunately, retinoic acid also plays a role in the development of cancerous tumors, making it often desirable to inhibit retinoic acid. You can inhibit retinoic acid by obtaining gene vectors that the cells of interest are known to take up, for genes that produce proteins that will prevent retinoic acid from binding to its receptors within cells.
Other People Are Reading
Instructions
-
-
1
Obtain a gene vector known to interfere with retinoic acid receptors (RARs). You can obtain these vectors from a science lab supplier.
-
2
Use a micropipette to transfer a given amount of the gene vector, recommended by the laboratory supplier, into a plate of cells. Swirl the plate around, in order to evenly distribute the vector throughout the plate.
-
-
3
Allow your cells to incubate with the vector for the next few days, so that they take up the vector and, accordingly, start producing proteins to interfere with RARs. Successful transfection with the gene vector will prevent retinoic acid from operating within your cells.
-
1
