How to Make Your Own Mycelium Jars


Mycelium is the branching structure of a fungus that draws nutrients and moisture to feed the part of the fungus that will bear spores, such as the portion of the mushroom most people are familiar with. If you think of it in terms of flowering plants, the mycelium is like the root that obtains food for the flower. To grow mushrooms at home, you can either purchase a commercial mushroom kit or you can start from scratch with a mycelium jar.

Things You'll Need

  • Petri dish of sterile agar
  • Psilocybe cubensis starter culture
  • Cotton swab, sterilized
  • 500 mL wide-mouth jar with screw top lid, sterilized
  • 130 grams sterilized rye grain
  • 100 mL water
  • Teaspoon, sterilized
  • Dip the cotton swab into the starter culture. Swipe the cotton swab across the agar.

  • Cover the petri dish and turn it upside down. Store it at 78 to 86 degrees Fahrenheit for one week. After a week, there should be a layer of white, stringy material. This is the mycelium inoculant.

  • Pour the rye grain and water into the jar. Allow to sit at room temperature overnight.

  • Use the teaspoon to scoop out a small chunk of the agar that has mycelium growing on it. The piece doesn't need to be more than half an inch square. Drop the piece into the jar.

  • Put the lid onto the jar and shake the jar to mix the contents. Loosen the lid enough to allow air into the jar.

  • Place the jar in a dark area where the temperature will remain at 78 to 85 degrees Fahrenheit. Every three days, tighten the lid and shake the jar. Loosen the lid again after each shaking. After about two weeks, the jar will be colonized with mycelium.

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