How to Invert a Microscope

Invert the microscope to have the light source above the object instead of below.

Compound light microscopes have the light source below the image. This allows the light to pass through the microscope slide so that you can view the magnified image. If you are trying to look at an object that can not fit on the microscope slide or that light cannot pass through, you should use an "inverted microscope," one in which the light source comes from above. If you do not have an inverted microscope, there are some small adjustments you can make to your compound microscope to allow you to view the object like an inverted microscope.

Things You'll Need

• Compound microscope
• Cardboard
• Two flexible lamps
• Petri dish

Instructions

Creating an Inverted Microscope from a Compound Microscope

1. Lower the stage of the microscope to the lowest point. Lower the light source as well if possible on your microscope.

2. Find a piece of sturdy cardboard. Cut the cardboard to fit over your stage to ensure the glass on the stage does not get broken or scratched.

3. Get two light sources, such as flexible lamps. Arrange the lamps to shine light from above the stage. One light source will work, but the more light you have, the greater the visibility.

4. Place the object on the stage. Look through the eyepiece and focus the object into clear view. Start with the 10X magnification. Depending on the size of your object, you can increase in magnification to 40X. Do not allow the object to touch the lens of the eyepiece. This can scratch and damage the eyepiece. If you are working with larger microorganisms, try using a small petri dish. If the object is too large, a dissecting scope or an actual inverted microscope may be necessary.

5. Adjust the direction of light from the flexible lamps if necessary. Color the cardboard black to enhance the image if you are having trouble viewing.

References

• Great Scopes:Activity: Viewing Solid Objects with your Compound Microscope