How do I Dilute Serum Glucose With Water?

Serum glucose needs to be diluted to make it experimentally useful.

Serum glucose is commonly used in laboratory experiments for determining the effect of substances or processes on blood sugar levels. Serum glucose is the closest approximation to the make-up of glucose contained in human plasma, and these experiments can be vital for aiding those suffering from diseases such as diabetes. As blood glucose levels are comparatively low in our blood, serum glucose needs to be diluted with water to make it experimentally useful.

Things You'll Need

• Pipette
• Test tube
• Test tube bung
• Test tube rack
• 10ml distilled water
• Serum glucose

Instructions

1. Using a pipette, measure out 9.9ml of distilled water into your test tube.

2. Draw up 0.1ml of serum glucose into your pipette and add this to the test tube.

3. Insert the test tube bung into your test tube, sealing it.

4. Shake the test tube vigorously and place it back into the test tube rack.

5. Wait for the liquid to settle. Your serum glucose/water solution is now 1:100.

References

• Rice University: Experimental Dilutions
• Health Test UK: Blood Glucose Test