If you want to find your blood type, buy a blood typing test kit and conduct the blood test at home by yourself. Alternatively, go to a clinic and undergo a blood test to find your blood type. Most labs use the "rapid blood agglutination" test to determine an individual's blood type. Your blood sample is tested against anti-A sera, anti-B sera and anti-Rh sera. The antigens present on your red blood cells may or may not react with the antibodies present in these sera.
Consequently, if there occurs clumping of red blood cells, then it indicates that your blood has reacted with one of the antibodies, which caused agglutination of your red blood cells due to the antigen-antibody reaction. The results are observed to ascertain your blood type.
Things You'll Need
- 1 clean microscope slide
- 1 wax pencil
- Anti-A sera
- Anti-B sera
- Anti-Rh sera
- Sterilized cotton
- 70 percent alcohol
- 1 sterile lancet
- 1 sterile plastic pipette
- 3 fresh toothpicks
Divide a clean microscope slide into three parts using wax pencil. Make three circles in each section of the slide. Label them as circle-A, circle-B and circle-Rh.
Add anti-A sera to circle-A, anti-B sera to circle-B and anti-Rh sera to circle-Rh. Leave aside the slide undisturbed.
Puncture your left middle finger with a sterile lancet after wiping your finger with a sterilized cotton ball that has been dipped in 70 percent alcohol or any other disinfectant solution.
Draw about three drops of blood into a sterile plastic pipette.
Add a drop of blood into each of the three circles from the sterile plastic pipette.
Mix the blood with the antibody sera carefully, using a fresh toothpick for each circle.
Allow the slide to air dry. Wait for five minutes and then observe all the three circles for agglutination. Agglutination in circle-A means your blood group is A, while agglutination in circle-B means your blood group is B. Agglutination in both circles A and B means your blood group is AB while absence of agglutination in both circles A and B means your blood group is O. Agglutination in circle-Rh indicates Rh (+) positive while absence of agglutination in circle-Rh indicates Rh (-) negative.
Tips & Warnings
- Be prudent while using antibody sera or adding blood to the circle. Too much quantity is difficult to manage and can ruin the test results. One drop of blood and one drop of antibody sera in enough.
- Tilting the slide can cause the antibody sera and blood to mix and ruin the results. Hence, place the slide on a flat surface and do not attempt to lift the slide until the test is over and the slide is dry.
- Air drying the slide is enough. Do not attempt to use a fan or a hair dryer to dry the slide.
- Discard the lancet, used cotton, plastic pipette as well as the toothpicks into a biohazard container. Wash the slide clean irrespective of whether you want to reuse it or discard it.
- While mixing the blood with the antibody sera using a toothpick, be careful to stay within the circle boundary. Rapid mixing can cause spilling or mixing of blood from one circle to the other.
- Photo Credit drawing blood image by Pix by Marti from Fotolia.com
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