How to Convert Lactose to Glucose

Lactose is a sugar found in the milk of all mammals. In order for lactose to be taken up by cells, it is first converted into glucose and galactose. This reaction occurs naturally in the gut of mammals. The lowering or loss of this lactase activity in adult animals can lead to lactose intolerance, as the lactose is left undigested and gut bacteria begin using lactose as a food supply. This produces gas, pain, and discomfort. This article will show how to convert lactose to glucose, which has a variety of uses at home and commercially.

Things You'll Need

  • Lactase (β-galactosidase) tablets
  • Glucose test strips
  • Milk -- any type (e.g. Whole, 2%, ect.)
  • Water
  • Timer
  • Measuring Cup
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Instructions

  1. Glucose Production from Milk Lactose

    • 1

      Crush the lactase tablet and add to 3/4 cup (200mL) of water. Stir until tablet has dissolved. Lactase tablets are available at most drug stores.

    • 2

      Dip the glucose test strip into the milk. It should test negative for glucose. The test strip is also available at most drug stores.

    • 3

      Add two parts milk (1 cup) to one part lactase solution (1/2 cup) and start the timer.

    • 4

      After two minutes, dip the glucose test strip into the milk lactase mixture. It should now test positive.

    • 5

      In the event the milk is still negative for glucose, stir the milk lactase mixture and allow the reaction to continue for 5-10 minutes and retest with the glucose test strip.

Tips & Warnings

  • If there is still no glucose in the solution after 10-15 minutes, the lactase tablets may be bad.

  • Do not leave lactase tablets in hot environments, as heat can render the enzyme inactive.

  • People with milk allergies should not handle or consume milk or milk products.

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