How to Make 40% Glucose Solution
Glucose has the molecular formula C6H12O6, making it a simple sugar. It is the main product of photosynthesis and is used widely used in organisms---including humans---as a source of chemical energy. A glucose solution consists of glucose mixed with a solvent, usually water. Students commonly prepare glucose solutions in classroom laboratories.
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Instructions
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Determine the desired weight of the glucose solution. This will typically be provided in the instructions for the experiment. For the purposes of this example, assume you want to prepare a 100g glucose solution.
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Calculate the mass of glucose needed for the solution. Concentration is defined as Mg/Ms = c, where Mg is the mass of the glucose, Ms is the total mass of the solution and c is the concentration. The total mass of the solution is 100g and the concentration is 40 percent, so Mg/Ms = c = Mg/100 = 0.4 --> Mg = (.4)(100) = 40g. You'll need 40g of glucose to make the solution.
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Pour the correct amount of water into the beaker. The mass of the solution must be 100g and, because the mass of the glucose is 40g, the mass of the water is 60g.
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Heat the water gently over a Bunsen burner. A 40-percent solution is close to the maximum concentration of 47-percent glucose that is possible at room temperature. Raising the temperature of the water will make the preparation of a 40-percent solution much easier.
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Add the 40g of glucose to the water. Stir thoroughly until the glucose completely dissolves.
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