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Step 1
Hold your rat gently yet firmly in your hands and turn it over so that it's belly is facing you.
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Step 2
Look at the belly and genital area. A male rat will have large testicles and a female rat will have nipples. Be aware that male rats will draw in their testicles when they get scared, so they may be hard to find.
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Step 3
Determine the sex of a rat as young as 10 days of age. Females will have small dots where the nipples will be and males will have a small bump where the testicles and penis will be located.
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Step 4
Observe a rat's behavior. This will also help you to determine their sex though this is not a fool-proof method. Male rats tend to be quieter and more affectionate than female rats, although males may go through an aggressive stage when they enter adolescence at around six months of age.








