Enzymes are proteins that catalyze, or affect the rate, of chemical reactions without themselves being altered in the process. Specific enzymes catalyze each cellular reaction.
The main role of enzymes during the respiration reaction is to assist in transferring electrons from one molecule to another. These transfers are called "redox" reactions, where the loss of electrons from one molecule (oxidation) must coincide with the addition of electrons to another substance (reduction).
This first step of the respiration reaction takes place in the cytoplasm, or fluid, of the cell. Glycolysis consists of nine separate chemical reactions, each catalyzed by a specific enzyme.
The key players in glycolysis are the enzyme dehydrodgenase and a coenzyme (non-protein helper) called NAD+. Dehydrodgenase oxidizes glucose by stripping two electrons from it and transferring them to NAD+. In the process glucose is "split" into two molecules of pyruvate, which continue the reaction.
The Citric Acid Cycle
The second step of the respiration reaction takes place inside a cell organelle called the mitochondria, which due to their role in ATP production are called "power factories" for the cell.
Just before the citric acid cycle starts, pyruvate is "groomed" for the reaction by being converted into a high-energy substance called acetyl coenzyme A, or acetyl-CoA.
Specific enzymes located in the mitochondria then power the many reactions that make up the citric acid cycle (also known as the Krebs cycle) by rearranging chemical bonds and participating in more redox reactions.
At the completion of this step, electron carrying molecules leave the citric acid cycle and begin the third step.
The final step of the respiration reaction, also called the electron transport chain, is where the energy payoff occurs for the cell. During this step oxygen drives a chain of electron movement across the membrane of the mitochondria. This transfer of electrons powers the ability of the enzyme ATP synthase to produce 38 molecules of ATP.
- Biology: Concepts and Connections; Neil A. Campbell; 2009
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