How do I Troubleshoot Autoclave?
An autoclave is a device for sterilizing equipment. High pressure saturated steam is applied to objects inside, usually at a temperature of 121 Celsius, and for 15 to 30 minutes. The heating, water and pressure elements of the autoclave must work correctly for proper sterilization.
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Water Issues
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Take steps to avoid excess water in the autoclave chamber. If items are wet after autoclaving, increase the drying time to eliminate excessive vapor in the chamber. A clogged drain may also result in excess water in the chamber after a completed run. Clean out the drain and make sure the drain line check valve is in working order and does not need replacement.
Heating Issues
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A high temperature is necessary for proper function. If the autoclave is not heating quickly enough, the thermal fuse may need replacement. Additionally, accumulated debris or a faulty chamber seal can cause a steam leak. Clean your autoclave and lubricate the seal using hand soap. Make sure you do not load too much into the autoclave; this may also slow the heating process.
If the boiler temperature is lower than necessary, there may be kinked exhaust tubing.
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Pressure Issues
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Make sure the autoclave is supplying sufficient pressure for effective sterilization. If the pressure is too high during the sterilization phase, there may be air trapped in the chamber due to a clogged strainer or drain. Clean the strainer and drain line and then flush them. An overcrowded autoclave and a faulty chamber seal can also result in insufficient pressure.
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References
- Photo Credit rain image by tomash from Fotolia.com Thermometer image by Ewe Degiampietro from Fotolia.com pressure manometer image by JoLin from Fotolia.com
