Cytospin Protocol
Cytospinning is a method used by microbiologists to get cells ready for microscopic viewing. Cytospin protocol is a step-by-step standardization of cytospinning procedures and is part of the process of biological and medical research worldwide.
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Cytospin Preparation
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First, dilute cells with phosphate buffered saline (PBS), or saline solution, with around 500 milliliters of solution for every 25,000 cells. Next, prepare the centrifuge by inserting, sealing and clamping slides, with the slides facing out, not in.
Cytospin Action
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Next, load cells and spin them at speed level 10 for four minutes, or level 13 for six minutes for smaller cells. After withdrawing the saline solution from each slide, add an equal amount of 4 percent pulverized fuel ash (PFA), a preservative, to preserve the cells. Next, repeat the spin cycle.
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Cytospin Cleanup
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Next, wash each slide with saline solution twice. Use a special pen called a "pap pen" to make squares around the cells. Finally, keep cells moist with more saline solution and let sit in a humidity chamber at 4 degrees Celsius until you need them.
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References
Resources
- Photo Credit microscope image by Fotocie from Fotolia.com
Comments
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anouuk
Jan 31, 2011
If you don't understand a scientific topic then that's fine; nobody can be an expert on everything. However, it really doesn't make sense writing a factually incorrect and misleading article about a topic outside your area of expertise and then posting it on a "how to" website that is supposedly written by people with expertise on a topic. It would probably have been better to have just posted a link to the protocol from the Tang Lab you referenced rather than getting the information incorrect while trying to rewrite their protocol here.
