3-D Point Spread Function

3-D Point Spread Function thumbnail
Point spread function is used to help reconstruct images in 3-D microscopy.

The point spread function (PSF) describes how an imaging system responds to a point object or a point source, or single identifiable source of an object. Point spread functions are used in three-dimensional (3-D) microscopy, an imaging technique.

  1. Definition

    • Point spread functions are the impulse response generated by an optical system. In terms of signal processing, the impulse response, or point spread function, of an optical system is defined as the output it produces when the system is presented with an impulse, or short input signal.

    3-D Microscopy

    • Point spread function used in 3-D microscopy involves a field of microscopy that utilizes microscopes to view objects and samples by scanning a beam over the surface of an object. The scanner takes a series of images of the objects at different focal depths, but from the same fixed position. Software then reconstructs, or recreates, a three-dimensional model based on the original sample taken.

    Point Spread Function

    • Images are specifically created in 3-D microscopy through the use of optical sensoring, which involves a point-by-point acquisition of an object via computer software. The 3-D microscope's imaging system scans the object and focuses on an image point, or where light rays originating from an object converges. The microscope creates impulses based on the point object, or point spread functions, which are later used in 3-D reconstruction.

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  • Photo Credit microscope image by Fotocie from Fotolia.com

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