Cell Migration Assay Protocol
Cell migration requires localized signaling events which cause changes in a cell's architecture. Cell migration is a cyclical process which makes a cell appear to "walk" by extending protrusions from the front and then retracting the other end.
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Types
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Migration assay protocols vary depending on the purpose of the research. Typical reasons for cell assay include the screening of pharmacological agents, evaluation of adhesion receptors, analysis of gene function and determination of protein involvement in the migration process.
Boyden Chamber Assay
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One of the most common cell migration assay protocols is the Boyden Chamber assay, which is used for quantitative research. A Boyden chamber consists of a cell culture insert, with a porous membrane lining, that is set inside a culture plate. Migratory cells then move through the porous membrane towards the well plate below, where they can be counted, stained and studied.
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Gap Closure Assays
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Reasearchers using Gap Closure assay protocol will be able to view migrating cells in action. The Gap Closure assay method creates an area for cells to move across, and observers can watch cell migration in real time with a microscope. Gap closure protocol can be used for both qualitative or quantitative research.
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References
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- Photo Credit cell culture image by alwayspp from Fotolia.com microscope kit image by PHOTOFLY from Fotolia.com
